Journal: Cells

Article Title: CPF Induces GC2 spd Cell Injury via ROS/AKT/Efcab6 Pathway

doi: 10.3390/cells14130940

Figure Lengend Snippet: Status of GC2 spd cells after CPF treatment for 24 h. ( A ) Phenotypes of GC2 spd cells; ( B ) cell number of GC2 spd cells; ( C ) cell viability of GC2 spd cells. Scale bar indicates 100 μm.

Article Snippet: The GC2 spd cell line was purchased from ATCC: The Global Bioresource Center ( https://www.atcc.org (accessed on 20 May 2025)) (CRL-2196, ATCC, Manassas, VA, USA).

Techniques:

Journal: Cells

Article Title: CPF Induces GC2 spd Cell Injury via ROS/AKT/Efcab6 Pathway

doi: 10.3390/cells14130940

Figure Lengend Snippet: Cell cycle arrest of GC2 spd cells after CPF treatment for 24 h. ( A ) Cell distributions of vehicle (DMSO) and CPF (25 μmol/L) group; ( B ) cell distributions of CPF (25 μmol/L) group; ( C ) statistics on cell cycle distribution; ( D ) mRNA expression of P53 ; ( E ) mRNA expression of P21 ; ( F ) mRNA expression of Ccna ; ( G ) mRNA expression of Ccnd ; ( H ) mRNA expression of Ccnb ; ( I ) mRNA expression of Ccne .

Article Snippet: The GC2 spd cell line was purchased from ATCC: The Global Bioresource Center ( https://www.atcc.org (accessed on 20 May 2025)) (CRL-2196, ATCC, Manassas, VA, USA).

Techniques: Expressing

Journal: Cells

Article Title: CPF Induces GC2 spd Cell Injury via ROS/AKT/Efcab6 Pathway

doi: 10.3390/cells14130940

Figure Lengend Snippet: Detection of apoptosis in GC2 spd cells after CPF treatment for 24 h. ( A ) TUNEL staining in GC2 spd cells; ( B ) TUNEL cell positivity rate in GC2 spd cells; ( C , D ) cell distributions of vehicle (DMSO) and CPF (25 μmol/L) group; ( E , F ) statistics of apoptotic cells in GC2 spd cells; ( G ) mRNA expression of Caspase3 ; ( H ) mRNA expression of Caspase9 . Blue fluorescence represents cells stained with DAPI, and green fluorescence indicates apoptotic cells. B1: membrane rupture cells; B2: represents late apoptotic cells and necrotic cells; B3: live cells; B4: early apoptotic cells. Scale bar indicates 100 μm. “ NS ” represents no significant difference between each group.

Article Snippet: The GC2 spd cell line was purchased from ATCC: The Global Bioresource Center ( https://www.atcc.org (accessed on 20 May 2025)) (CRL-2196, ATCC, Manassas, VA, USA).

Techniques: TUNEL Assay, Staining, Expressing, Fluorescence, Membrane

Journal: Cells

Article Title: CPF Induces GC2 spd Cell Injury via ROS/AKT/Efcab6 Pathway

doi: 10.3390/cells14130940

Figure Lengend Snippet: Mitochondrial homeostasis assay in GC2 spd cells after 24 h of CPF treatment. ( A ) ROS levels in GC2 spd cells; ( B ) expression of Vdac1 ; ( C ) expression of Cox4 . “ NS ” represents no significant difference between each group.

Article Snippet: The GC2 spd cell line was purchased from ATCC: The Global Bioresource Center ( https://www.atcc.org (accessed on 20 May 2025)) (CRL-2196, ATCC, Manassas, VA, USA).

Techniques: Expressing

Journal: Cells

Article Title: CPF Induces GC2 spd Cell Injury via ROS/AKT/Efcab6 Pathway

doi: 10.3390/cells14130940

Figure Lengend Snippet: Analysis of RNA sequencing data for GC2 spd cells after CPF treatment for 24 h. ( A ) Sample expression violin plot; ( B ) principal component analysis of sample; ( C ) sample correlation heat map; ( D ) statistical chart of differential genes; pink dots represent significantly up-regulated genes, blue dots represent significantly down-regulated genes, and the rest are non-significant genes. ( E ) GO enrichment classification histogram; ( F ) KEGG enrichment bubble plot; ( G ) Reactome enrichment bubble chart. Data presented are from four independent experiments ( n = 4).

Article Snippet: The GC2 spd cell line was purchased from ATCC: The Global Bioresource Center ( https://www.atcc.org (accessed on 20 May 2025)) (CRL-2196, ATCC, Manassas, VA, USA).

Techniques: RNA Sequencing, Expressing

Journal: Cells

Article Title: CPF Induces GC2 spd Cell Injury via ROS/AKT/Efcab6 Pathway

doi: 10.3390/cells14130940

Figure Lengend Snippet: Restorative effect of NAC on GC2 spg cells after CPF treatment for 24 h. ( A – L ) Restorative effect of NAC on GC2 spg cells after 12.5 μmol/L CPF exposure; ( A ′– L′ ) restorative effect of NAC on GC2 spg cells after 25 μmol/L CPF exposure; ( A , A′ ) phenotypes of GC2 spd cells; scale bar indicates 100 μm. ( B , B′ ) cell viability of GC2 spd cells; ( C , C′ ) cell number of GC2 spd cells; ( D , D′ ) level of ROS in GC2 spd cells; ( E , E′ ) expression of Bax ; ( F , F′ ) expression of Pcna ; ( G , G′ ) expression of P21 ; ( H , H′ ) expression of Ccnd ; ( I , I′ ) expression of Ccna ; ( J , J′ ) expression of Ccne ; ( K , K′ ) expression of Ccnb ; ( L , L′ ) expression of Efcab6 . Scale bar indicates 100 μm.

Article Snippet: The GC2 spd cell line was purchased from ATCC: The Global Bioresource Center ( https://www.atcc.org (accessed on 20 May 2025)) (CRL-2196, ATCC, Manassas, VA, USA).

Techniques: Expressing